Application
SRD5A3 Antibody (C-term)?),293(lane 2) cell line lysates (35ug/lane). 鑱絋his demonstrates the SRD5A3 antibody detected the SRD5A3 protein (arrow).Application
SRD5A3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SRD5A3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SRD5A3 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SRD5A3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRD5A3; SRD5A2L; Probable polyprenol reductase; 3-oxo-5-alpha-steroid 4-dehydrogenase 3; Steroid 5-alpha-reductase 2-like; Steroid 5-alpha-reductase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SRD5A2L |
|---|---|
| Alternative Names | SRD5A3 SRD5A2L Probable polyprenol reductase 3-oxo-5-alpha-steroid 4-dehydrogenase 3 Steroid 5-alpha-reductase 2-like Steroid 5-alpha-reductase 3 |
| Molecular Weight(MW) | 36521 Da |
| Function | Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT) |
| Tissue Specificity | Overexpressed in hormone-refractory prostate cancers (HRPC). Almost no or little expression in normal adult organs |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable) |
| Entrez Gene | 79644 |
|---|
Application
SRD5A3 Antibody (C-term)?),293(lane 2) cell line lysates (35ug/lane). 鑱絋his demonstrates the SRD5A3 antibody detected the SRD5A3 protein (arrow).
Application
SRD5A3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SRD5A3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SRD5A3 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
