Application
Western blot analysis of SRF Antibody (N-term) in HepG2 cell line lysates (35ug/lane). SRF (arrow) was detected using the purified Pab.Application
SRF Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SRF Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRF; Serum response factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SRF Serum response factor |
|---|---|
| Molecular Weight(MW) | 51593 Da |
| Function | SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation |
| Tissue Specificity | This SRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135~165 amino acids from the N-terminal region of human SRF. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6722 |
|---|
Application
Western blot analysis of SRF Antibody (N-term) in HepG2 cell line lysates (35ug/lane). SRF (arrow) was detected using the purified Pab.
Application
SRF Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
