Application
SRGN Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SRGN antibody detected the SRGN protein (arrow).Application
SRGN Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SRGN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRGN; PRG; PRG1; Serglycin; Hematopoietic proteoglycan core protein; Platelet proteoglycan core protein; Secretory granule proteoglycan core protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRG PRG1 |
|---|---|
| Alternative Names | SRGN PRG PRG1 Serglycin Hematopoietic proteoglycan core protein Platelet proteoglycan core protein Secretory granule proteoglycan core protein |
| Molecular Weight(MW) | 17652 Da |
| Function | Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF- alpha and may also regulate protease secretion. Inhibits bone mineralization |
| Tissue Specificity | This SRGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-148 amino acids from the C-terminal region of human SRGN. |
| Cellular Localization | Cytoplasmic granule (By similarity). Secreted, extracellular space. Golgi apparatus. Note=Found in mast cell granules and in cytoplasmic granules of cytolytic T lymphocytes from where it is secreted upon cell activation Secreted constitutively by endothelial cells and macrophages Located to Golgi apparatus during neutrophil differentiation |
| Entrez Gene | 5552 |
|---|---|
| Nucleotide Accession | NP_002718.2 |
Application
SRGN Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SRGN antibody detected the SRGN protein (arrow).
Application
SRGN Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
