Application
Western blot analysis of SSB3 antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). SSB3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SSB3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using SSB3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SSB3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPSB3; C16orf31; SSB3; SPRY domain-containing SOCS box protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C16orf31 SSB3 |
|---|---|
| Alternative Names | SPSB3 C16orf31 SSB3 SPRY domain-containing SOCS box protein 3 |
| Molecular Weight(MW) | 39376 Da |
| Function | May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity) |
| Tissue Specificity | This SSB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162~191 amino acids from the Center region of human SSB3. |
| Entrez Gene | 90864 |
|---|
Application
Western blot analysis of SSB3 antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). SSB3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with SSB3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using SSB3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
