Application
SSRP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SSRP1 antibody detected the SSRP1 protein (arrow).| Product Name | SSRP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SSRP1; FACT80; FACT complex subunit SSRP1; Chromatin-specific transcription elongation factor 80 kDa subunit; Facilitates chromatin transcription complex 80 kDa subunit; Facilitates chromatin transcription complex subunit SSRP1; Recombination signal sequen |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FACT80 |
|---|---|
| Alternative Names | SSRP1 FACT80 FACT complex subunit SSRP1 Chromatin-specific transcription elongation factor 80 kDa subunit Facilitates chromatin transcription complex 80 kDa subunit Facilitates chromatin transcription complex subunit SSRP1 Recombination signal sequen |
| Molecular Weight(MW) | 81075 Da |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63 |
| Tissue Specificity | This SSRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-518 amino acids from the C-terminal region of human SSRP1. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci |
| Entrez Gene | 6749 |
|---|---|
| Nucleotide Accession | NP_003137.1 |
Application
SSRP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SSRP1 antibody detected the SSRP1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
