Application
A. Western blot analysis on (A) 293T and (B) SW480 cell lysates using anti-ST13/HIP RabMAb (Cat. #AJ1736a), dilution 1:100,000.Application
B.Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-ST13/HIP RabMAb (Cat. #AJ1736a).Application
C. Immunohistochemical analysis of paraffin-embedded human normal colon tissue using anti-ST13/HIP RabMAb (Cat. #AJ1736a).| Product Name | ST13/HIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCG1, PLC1, 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC-II;PLC-148 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP3264 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AAG2 FAM10A1 HIP SNC6 |
|---|---|
| Alternative Names | PLCG1 PLC1 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 PLC-II PLC-148 |
| Molecular Weight(MW) | 41332 Da |
| Function | One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminus of human Hsc70 Interacting Protein/HIP was used as an immunogen. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 6767 |
|---|
Application
A. Western blot analysis on (A) 293T and (B) SW480 cell lysates using anti-ST13/HIP RabMAb (Cat. #AJ1736a), dilution 1:100,000.
Application
B.Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-ST13/HIP RabMAb (Cat. #AJ1736a).
Application
C. Immunohistochemical analysis of paraffin-embedded human normal colon tissue using anti-ST13/HIP RabMAb (Cat. #AJ1736a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
