Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ST13 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-ST13 Antibody (N-term) pre-incubated with and without blocking peptide (BP6247a) in A2058 cell line lysate. ST13(arrow) was detected using the purified Pab (1:240 dilution).| Product Name | HSC70 Interacting Protein HIP (ST13) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ST13; FAM10A1; HIP; SNC6; Hsc70-interacting protein; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; Suppression of tumorigenicity 13 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AAG2 FAM10A1 HIP SNC6 |
|---|---|
| Alternative Names | ST13 FAM10A1 HIP SNC6 Hsc70-interacting protein Progesterone receptor-associated p48 protein Protein FAM10A1 Putative tumor suppressor ST13 Renal carcinoma antigen NY-REN-33 Suppression of tumorigenicity 13 protein |
| Molecular Weight(MW) | 41332 Da |
| Function | One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity) |
| Tissue Specificity | This HSC70 Interacting Protein HIP (ST13) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~87 amino acids from the N-terminal region of human ST13. |
| Cellular Localization | Cytoplasm (By similarity). |
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ST13 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-ST13 Antibody (N-term) pre-incubated with and without blocking peptide (BP6247a) in A2058 cell line lysate. ST13(arrow) was detected using the purified Pab (1:240 dilution).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
