Application
Western blot analysis of ST8SIA2 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). ST8SIA2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue with ST8SIA2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ST8SIA2 Antibody (N-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ST8SIA2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ST8SIA2; SIAT8B; STX; Alpha-2, 8-sialyltransferase 8B; Sialyltransferase 8B; Sialyltransferase X; Sialytransferase St8Sia II |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIAT8B STX |
|---|---|
| Alternative Names | ST8SIA2 SIAT8B STX Alpha-2 8-sialyltransferase 8B Sialyltransferase 8B Sialyltransferase X Sialytransferase St8Sia II |
| Molecular Weight(MW) | 42430 Da |
| Function | May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression |
| Tissue Specificity | Highly expressed in fetal brain, kidney and heart and to a much lesser extent in adult heart and thymus |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (Potential) |
| Entrez Gene | 8128 |
|---|
Application
Western blot analysis of ST8SIA2 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). ST8SIA2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue with ST8SIA2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ST8SIA2 Antibody (N-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
