Application
Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP18086b STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).Application
STAT3 Antibody (C-term S727) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).| Product Name | STAT3 Antibody (C-term S727) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APRF |
|---|---|
| Alternative Names | STAT3 APRF Signal transducer and activator of transcription 3 Acute-phase response factor |
| Molecular Weight(MW) | 88068 Da |
| Function | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA |
| Tissue Specificity | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 Identified in a complex with LYN and PAG1 |
| Entrez Gene | 6774 |
|---|---|
| Nucleotide Accession | NP_003141.2 |
Application
Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP18086b STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).
Application
STAT3 Antibody (C-term S727) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
