Application
STAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of STAT2 Antibody (C-term) (Cat.# AP1532a) in K562 cell line lysates (35ug/lane). STAT2(arrow) was detected using the purified Pab.| Product Name | STAT2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT2; Signal transducer and activator of transcription 2; p113 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | STAT2 Signal transducer and activator of transcription 2 p113 |
|---|---|
| Molecular Weight(MW) | 97916 Da |
| Function | Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta) Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state |
| Tissue Specificity | This STAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 822~851 amino acids from the C-terminal region of human STAT2. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta |
| Entrez Gene | 6773 |
|---|
Application
STAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of STAT2 Antibody (C-term) (Cat.# AP1532a) in K562 cell line lysates (35ug/lane). STAT2(arrow) was detected using the purified Pab.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
