Application
The anti-STK16 Pab is used in Western blot to detect STK16 in mouse brain tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of STK16 (arrow) using rabbit polyclonal STK16 Antibody (S32) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STK16 gene.| Product Name | STK16 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STK16; MPSK1; PKL12; TSF1; Serine/threonine-protein kinase 16; Myristoylated and palmitoylated serine/threonine-protein kinase; Protein kinase PKL12; TGF-beta-stimulated factor 1; Tyrosine-protein kinase STK16; hPSK |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MPSK1 PKL12 TSF1 |
|---|---|
| Alternative Names | STK16 MPSK1 PKL12 TSF1 Serine/threonine-protein kinase 16 Myristoylated and palmitoylated serine/threonine-protein kinase Protein kinase PKL12 TGF-beta-stimulated factor 1 Tyrosine-protein kinase STK16 hPSK |
| Molecular Weight(MW) | 34656 Da |
| Function | Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins |
| Tissue Specificity | Ubiquitously expressed at very low levels. |
| Cellular Localization | Cytoplasm, perinuclear region. Membrane; Lipid-anchor (By similarity). Note=Associates with Golgi and Golgi-derived vesicles (By similarity) |
| Entrez Gene | 8576 |
|---|
Application
The anti-STK16 Pab is used in Western blot to detect STK16 in mouse brain tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of STK16 (arrow) using rabbit polyclonal STK16 Antibody (S32) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STK16 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
