Application
Western blot analysis of anti-CHIP(STUB1) Pab in T47D cell lysate. CHIP(STUB1)(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
CHIP(STUB1) Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow).| Product Name | STUB1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STUB1; CHIP; E3 ubiquitin-protein ligase CHIP; Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; STIP1 homology and U box-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CHIP |
|---|---|
| Alternative Names | STUB1 CHIP E3 ubiquitin-protein ligase CHIP Antigen NY-CO-7 CLL-associated antigen KW-8 Carboxy terminus of Hsp70-interacting protein STIP1 homology and U box-containing protein 1 |
| Molecular Weight(MW) | 34856 Da |
| Function | E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4 Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation |
| Tissue Specificity | Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung |
| Cellular Localization | Cytoplasm. |
Application
Western blot analysis of anti-CHIP(STUB1) Pab in T47D cell lysate. CHIP(STUB1)(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
CHIP(STUB1) Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
