Application
A. Western blot analysis on LnCaP cell lysate using anti-SuFu RabMAb (Cat. #AJ1744a), dilution 1:200.Application
B. Immunohistochemical staining of paraffin-embedded human fetal kidneys using anti-SuFu RabMAb (Cat. #AJ1744a).| Product Name | SUFU Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUFU, Suppressor of fused homolog |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1207Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SUFU Suppressor of fused homolog |
|---|---|
| Molecular Weight(MW) | 53947 Da |
| Function | Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU- GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A) |
| Tissue Specificity | Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 51684 |
|---|
Application
A. Western blot analysis on LnCaP cell lysate using anti-SuFu RabMAb (Cat. #AJ1744a), dilution 1:200.
Application
B. Immunohistochemical staining of paraffin-embedded human fetal kidneys using anti-SuFu RabMAb (Cat. #AJ1744a).| Application Notes | WB:1:10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
