Application
Western blot analysis of SUMF1 Antibody (C-Term) in Hela cell line lysates (35ug/lane). SUMF1 (arrow) was detected using the purified Pab.Application
SUMF1 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SUMF1 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
SUMF1 Antibody (C-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SUMF1 Antibody (C-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMF1; FGE; Sulfatase-modifying factor 1; C-alpha-formylglycine-generating enzyme 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FGE |
|---|---|
| Alternative Names | SUMF1 FGE Sulfatase-modifying factor 1 C-alpha-formylglycine-generating enzyme 1 |
| Molecular Weight(MW) | 40556 Da |
| Function | Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE |
| Tissue Specificity | Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart |
| Cellular Localization | Endoplasmic reticulum lumen. |
| Entrez Gene | 285362 |
|---|
Application
Western blot analysis of SUMF1 Antibody (C-Term) in Hela cell line lysates (35ug/lane). SUMF1 (arrow) was detected using the purified Pab.
Application
SUMF1 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SUMF1 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
SUMF1 Antibody (C-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
