Application
The SUMO1 monoclonal antibody (Cat. #AM1200a) is used in Western blot to detect SUMO1 in HL60 cell lysate.| Product Name | SUMO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO1; SMT3C; SMT3H3; UBL1; Small ubiquitin-related modifier 1; GAP-modifying protein 1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 66AT1273.94 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMT3C SMT3H3 UBL1 |
|---|---|
| Alternative Names | SUMO1 SMT3C SMT3H3 UBL1 Small ubiquitin-related modifier 1 GAP-modifying protein 1 SMT3 homolog 3 Sentrin Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein SMT3C Ubiquitin-like protein UBL1 |
| Molecular Weight(MW) | 11557 Da |
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2 Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development |
| Tissue Specificity | Purified recombinant GST-SUMO1 fusion protein was used as immunogen. |
| Cellular Localization | Nucleus membrane. Nucleus speckle. Cytoplasm. Note=Recruited by BCL11A into the nuclear body (By similarity) |
| Entrez Gene | 7341 |
|---|
Application
The SUMO1 monoclonal antibody (Cat. #AM1200a) is used in Western blot to detect SUMO1 in HL60 cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
