Application
The anti-SUMO2/3 Pab is used in Western blot to detect SUMO2/3 in Saos-2 cell lysate.Application
Western blot analysis of SUMO3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SUMO3 gene (Lane 2) (Origene Technologies).| Product Name | SUMO2/3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMT3B SMT3H1 |
|---|---|
| Alternative Names | SUMO3 SMT3B SMT3H1 Small ubiquitin-related modifier 3 SMT3 homolog 1 SUMO-2 Ubiquitin-like protein SMT3B |
| Molecular Weight(MW) | 11637 Da |
| Function | Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 |
| Tissue Specificity | Expressed predominantly in liver. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 6612 |
|---|
Application
The anti-SUMO2/3 Pab is used in Western blot to detect SUMO2/3 in Saos-2 cell lysate.
Application
Western blot analysis of SUMO3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SUMO3 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
