Application
Western blot analysis of anti-SUMO2/3 Antibody (C-term E69) in CEM cell line lysates (35ug/lane).SUMO2/3(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (C-term E69) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.| Product Name | SUMO2/3 Antibody (C-term E69) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMT3B SMT3H1 |
|---|---|
| Alternative Names | SUMO3 SMT3B SMT3H1 Small ubiquitin-related modifier 3 SMT3 homolog 1 SUMO-2 Ubiquitin-like protein SMT3B |
| Molecular Weight(MW) | 11637 Da |
| Function | Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 |
| Tissue Specificity | Expressed predominantly in liver. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 6612 |
|---|
Application
Western blot analysis of anti-SUMO2/3 Antibody (C-term E69) in CEM cell line lysates (35ug/lane).SUMO2/3(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (C-term E69) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
