Application
Dot blot analysis of anti-hSUMO4-V55 MT specific Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Confocal immunofluorescent analysis of SUMO4-V55 MT specific Antibody(Cat#AP3729a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | SUMO4-V55 MT specific Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO4; SMT3H4; Small ubiquitin-related modifier 4; Small ubiquitin-like protein 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIF |
| DB:1:500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMT3H4 |
|---|---|
| Alternative Names | SUMO4 SMT3H4 Small ubiquitin-related modifier 4 Small ubiquitin-like protein 4 |
| Molecular Weight(MW) | 10685 Da |
| Function | Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I |
| Tissue Specificity | Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen |
| Entrez Gene | 387082 |
|---|---|
| Nucleotide Accession | NP_001002255 |
Application
Dot blot analysis of anti-hSUMO4-V55 MT specific Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Confocal immunofluorescent analysis of SUMO4-V55 MT specific Antibody(Cat#AP3729a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | DB:1:500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step pecific peptide affinity purification. |
