Application
SUPT16H Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SUPT16H antibody detected the SUPT16H protein (arrow).Application
SUPT16H Antibody (Center) (AP13844c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SUPT16H Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | SUPT16H Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUPT16H; FACT140; FACTP140; FACT complex subunit SPT16; Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FACT140 FACTP140 |
|---|---|
| Alternative Names | SUPT16H FACT140 FACTP140 FACT complex subunit SPT16 Chromatin-specific transcription elongation factor 140 kDa subunit FACT 140 kDa subunit FACTp140 Facilitates chromatin transcription complex subunit SPT16 |
| Molecular Weight(MW) | 119914 Da |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci |
| Entrez Gene | 11198 |
|---|---|
| Nucleotide Accession | NP_009123.1 |
Application
SUPT16H Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SUPT16H antibody detected the SUPT16H protein (arrow).
Application
SUPT16H Antibody (Center) (AP13844c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SUPT16H Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
