Application
SUPT5H Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SUPT5H antibody detected the SUPT5H protein (arrow).| Product Name | SUPT5H Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUPT5H; SPT5; SPT5H; Transcription elongation factor SPT5; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; Tat-cotransactivator 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPT5 SPT5H |
|---|---|
| Alternative Names | SUPT5H SPT5 SPT5H Transcription elongation factor SPT5 DRB sensitivity-inducing factor 160 kDa subunit DRB sensitivity-inducing factor large subunit Tat-cotransactivator 1 protein |
| Molecular Weight(MW) | 121000 Da |
| Function | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6829 |
|---|---|
| Nucleotide Accession | NP_001104490.1 NP_001124297.1 NP_001124296.1 |
Application
SUPT5H Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SUPT5H antibody detected the SUPT5H protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
