Application
A. Western blot analysis on mouse brain lysates using anti-Synapsin (pS553) RabMAb (Cat. #AJ1749a), dilution 1:50,000Application
B. Immunofluorescent staining of PC-12 cells using anti-Synapsin (pS553) RabMAb (Cat. #AJ1749a)Application
C. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Synapsin (pS553) RabMAb (Cat. #AJ1749a) dilution 1:500| Product Name | Synapsin I Antibody Phospho (pS553) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYN1, Synapsin-1, Synapsin I;Brain protein 4.1 |
| Modification | p-S553 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E377 |
| Host Species | Rabbit |
| Tested Applications | WBIFDB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SYN1 Synapsin-1 Synapsin I Brain protein 4.1 |
|---|---|
| Molecular Weight(MW) | 74111 Da |
| Function | Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Ser553 of human Synapsin was used as immunogen. The antibody only detects Synapsin phosphorylated on Serine 553. |
| Cellular Localization | Cell junction, synapse. Golgi apparatus (By similarity) |
| Entrez Gene | 6853 |
|---|
Application
A. Western blot analysis on mouse brain lysates using anti-Synapsin (pS553) RabMAb (Cat. #AJ1749a), dilution 1:50,000
Application
B. Immunofluorescent staining of PC-12 cells using anti-Synapsin (pS553) RabMAb (Cat. #AJ1749a)
Application
C. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Synapsin (pS553) RabMAb (Cat. #AJ1749a) dilution 1:500| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
