Application
(LEFT) Western blot analysis of TAF2 Antibody (C-Term) in MCF-7 cell line lysates (35ug/lane). TAF2 (arrow) was detected using the purified Pab.(RIGHT) Western blot analysis of TAF2 Antibody (C-Term) in mouse NIH-3T3 cell line lysates (35ug/lane). TAF2 (arrow) was detected using the purified Pab.Application
TAF2 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF2 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TAF2 Antibody (C-Term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TAF2 Antibody (C-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAF2; CIF150; TAF2B; Transcription initiation factor TFIID subunit 2; 150 kDa cofactor of initiator function; RNA polymerase II TBP-associated factor subunit B; TBP-associated factor 150 kDa; Transcription initiation factor TFIID 150 kDa subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CIF150 TAF2B |
|---|---|
| Alternative Names | TAF2 CIF150 TAF2B Transcription initiation factor TFIID subunit 2 150 kDa cofactor of initiator function RNA polymerase II TBP-associated factor subunit B TBP-associated factor 150 kDa Transcription initiation factor TFIID 150 kDa subunit |
| Molecular Weight(MW) | 136971 Da |
| Function | Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter |
| Tissue Specificity | Expressed in all tissues tested. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6873 |
|---|
Application
(LEFT) Western blot analysis of TAF2 Antibody (C-Term) in MCF-7 cell line lysates (35ug/lane). TAF2 (arrow) was detected using the purified Pab.(RIGHT) Western blot analysis of TAF2 Antibody (C-Term) in mouse NIH-3T3 cell line lysates (35ug/lane). TAF2 (arrow) was detected using the purified Pab.
Application
TAF2 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF2 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TAF2 Antibody (C-Term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
