Application
Western blot analysis of TAGLN antibody (N-term) in SK-Br-3 cell line lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-MB468 cells using TAGLN Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TAGLN Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAGLN; SM22; WS3-10; Transgelin; 22 kDa actin-binding protein; Protein WS3-10; Smooth muscle protein 22-alpha |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:50~100 IHC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SM22 WS3-10 |
|---|---|
| Alternative Names | TAGLN SM22 WS3-10 Transgelin 22 kDa actin-binding protein Protein WS3-10 Smooth muscle protein 22-alpha |
| Molecular Weight(MW) | 22611 Da |
| Function | Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence |
| Tissue Specificity | This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30~60 amino acids from the N-terminal region of human TAGLN. |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 6876 |
|---|
Application
Western blot analysis of TAGLN antibody (N-term) in SK-Br-3 cell line lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-MB468 cells using TAGLN Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
