Application
TARBP2 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the TARBP2 antibody detected the TARBP2 protein (arrow).Application
TARBP2 Antibody (N-term) (AP14108a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TARBP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TARBP2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TARBP2; TRBP; RISC-loading complex subunit TARBP2; TAR RNA-binding protein 2; Trans-activation-responsive RNA-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TRBP |
|---|---|
| Alternative Names | TARBP2 TRBP RISC-loading complex subunit TARBP2 TAR RNA-binding protein 2 Trans-activation-responsive RNA-binding protein |
| Molecular Weight(MW) | 39039 Da |
| Function | Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre- miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2 EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR |
| Tissue Specificity | This TARBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-68 amino acids from the N-terminal region of human TARBP2. |
| Cellular Localization | Cytoplasm. Cytoplasm, perinuclear region. Nucleus |
| Entrez Gene | 6895 |
|---|---|
| Nucleotide Accession | NP_599151.2 NP_599150.1 |
Application
TARBP2 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the TARBP2 antibody detected the TARBP2 protein (arrow).
Application
TARBP2 Antibody (N-term) (AP14108a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TARBP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
