Application
Western blot analysis of TBP antibody (Center) in HL60 cell line lysates (35ug/lane). TBP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TBP Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of TBP Antibody (Center)(Cat#AP6680c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | TBP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GTF2D1 TF2D TFIID |
|---|---|
| Alternative Names | TBP GTF2D1 TF2D TFIID TATA-box-binding protein TATA sequence-binding protein TATA-binding factor TATA-box factor Transcription initiation factor TFIID TBP subunit |
| Molecular Weight(MW) | 37698 Da |
| Function | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA |
| Tissue Specificity | Widely expressed, with levels highest in the testis and ovary |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6908 |
|---|
Application
Western blot analysis of TBP antibody (Center) in HL60 cell line lysates (35ug/lane). TBP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TBP Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of TBP Antibody (Center)(Cat#AP6680c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
