Application
TBP Antibody (Cat. #AM2196b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).Application
TBP Antibody (Cat. #AM2196b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).| Product Name | TBP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBP;GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA-box-binding protein; TATA sequence-binding protein; TATA-box-binding protein; TATA-binding factor; TATA-box-binding protein; TATA-box factor; TATA-box-binding protein; Transcription initiation factor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 830CT4.3.3 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GTF2D1 TF2D TFIID |
|---|---|
| Alternative Names | TBP GTF2D1 TF2D TFIID TATA-box-binding protein TATA-box-binding protein TATA sequence-binding protein TATA-box-binding protein TATA-binding factor TATA-box-binding protein TATA-box factor TATA-box-binding protein Transcription initiation factor |
| Molecular Weight(MW) | 37698 Da |
| Function | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA |
| Tissue Specificity | Widely expressed, with levels highest in the testis and ovary |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6908 |
|---|
Application
TBP Antibody (Cat. #AM2196b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).
Application
TBP Antibody (Cat. #AM2196b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TBP antibody detected the TBP protein (arrow).| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
