Application
TBX6 Antibody (Center) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).Application
Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center)(Cat#AP12063c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.| Product Name | TBX6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TBX6; T-box transcription factor TBX6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TBX6 T-box transcription factor TBX6 |
|---|---|
| Molecular Weight(MW) | 47045 Da |
| Function | T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to play also an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia (By similarity) |
| Tissue Specificity | Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 6911 |
|---|---|
| Nucleotide Accession | NP_004599.2 |
Application
TBX6 Antibody (Center) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).
Application
Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center)(Cat#AP12063c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
