Application
A. Western blot analysis on Jurkat cell lysate using anti-TCF4 RabMAb (Cat. #AJ1760a), dilution 1:100,000Application
B. Immunofluorescent staining of HeLa cells using anti-TCF-4 RabMAb (Cat. #AJ1760a).| Product Name | TCF-4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF7L2, TCF4, Transcription factor 7-like 2, HMG box transcription factor 4;T-cell-specific transcription factor 4 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2033Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TCF4 |
|---|---|
| Alternative Names | TCF7L2 TCF4 Transcription factor 7-like 2 HMG box transcription factor 4 T-cell-specific transcription factor 4 |
| Molecular Weight(MW) | 67919 Da |
| Function | Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1 Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine |
| Tissue Specificity | Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom |
| Cellular Localization | Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies |
| Entrez Gene | 6934 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-TCF4 RabMAb (Cat. #AJ1760a), dilution 1:100,000
Application
B. Immunofluorescent staining of HeLa cells using anti-TCF-4 RabMAb (Cat. #AJ1760a).| Application Notes | WB:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM222702