Application
Antibody used in WB on HepG2 at 0.2-1 ug/ml.Application
Antibody used in WB on human neuroblastoma at 1:100.| Product Name | TCF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCF4, E2-2, ITF2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, PTHS, ITF-2, SEF-2, TCF-4, SEF2-1D, bHLHb19 |
| Product description | TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts; however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.1) Raurell, I., (2006) J. Biol. Chem. 281 (3), 1401-1411. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TCF4 antibody can be used for detection of TCF4 by ELISA at 1:62500. TCF4 antibody can be used for detection of TCF4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TCF4 |
|---|---|
| Alternative Names | TCF4 E2-2 ITF2 SEF2 SEF2-1 SEF2-1A SEF2-1B PTHS ITF-2 SEF-2 TCF-4 SEF2-1D bHLHb19 |
| Molecular Weight(MW) | 71 kDa |
Application
Antibody used in WB on HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on human neuroblastoma at 1:100.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TCF4 antibody can be used for detection of TCF4 by ELISA at 1:62500. TCF4 antibody can be used for detection of TCF4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TCF4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM292986