Application
Figure 1: Western blot analysis using TCF3 mouse mAb against A549 (1), A431 (2), Hela (3), PANC-1 (4) and PC-3 (5) cell lysate.Application
Figure 2: Flow cytometric analysis of A549 cells using anti-TCF3 mAb (green) and negative control (purple).| Product Name | TCF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2A; ITF1; VDIR; bHLHb21; MGC129647; MGC129648; TCF3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6B8 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500 - 2000. FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB21 E2A ITF1 |
|---|---|
| Alternative Names | E2A ITF1 VDIR bHLHb21 MGC129647 MGC129648 TCF3 |
| Molecular Weight(MW) | 67600 Da |
| Function | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue- specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E- box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region |
| Tissue Specificity | Purified recombinant fragment of human TCF3 expressed in E. Coli. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6929 |
|---|---|
| Nucleotide Accession | NP_003191.1 |
Application
Figure 1: Western blot analysis using TCF3 mouse mAb against A549 (1), A431 (2), Hela (3), PANC-1 (4) and PC-3 (5) cell lysate.
Application
Figure 2: Flow cytometric analysis of A549 cells using anti-TCF3 mAb (green) and negative control (purple).| Application Notes | WB:1:500 - 2000. FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human TCF3 expressed in E. Coli. |
