Application
Western blot analysis of TCHP Antibody (Center) in CEM cell line lysates (35ug/lane). TCHP (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with TCHP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TCHP Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TCHP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCHP; Trichoplein keratin filament-binding protein; Mitochondrial protein with oncostatic activity; Tumor suppressor protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TCHP Trichoplein keratin filament-binding protein Mitochondrial protein with oncostatic activity Tumor suppressor protein |
|---|---|
| Molecular Weight(MW) | 61072 Da |
| Function | Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells |
| Tissue Specificity | Expressed at high levels in normal urothelial and breast epithelial cells. Also expressed in the smooth muscle and endothelial cells. Reduced expression seen in advanced bladder and breast carcinomas (at protein level). Ubiquitous. Expressed at highest levels in the heart, skeletal muscle, kidney, liver and testis |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm. Cell membrane. Mitochondrion |
| Entrez Gene | 84260 |
|---|
Application
Western blot analysis of TCHP Antibody (Center) in CEM cell line lysates (35ug/lane). TCHP (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with TCHP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TCHP Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
