Application
Western blot analysis of TDG antibody (C-term) in K562 cell line lysates (35ug/lane). TDG (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of K562 cells using TDG Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TDG Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TDG; G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TDG G/T mismatch-specific thymine DNA glycosylase Thymine-DNA glycosylase |
|---|---|
| Molecular Weight(MW) | 46053 Da |
| Function | In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine |
| Tissue Specificity | This TDG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288~318 amino acids from the C-terminal region of human TDG. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6996 |
|---|
Application
Western blot analysis of TDG antibody (C-term) in K562 cell line lysates (35ug/lane). TDG (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of K562 cells using TDG Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
