Application
Western blot analysis of TECT1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). TECT1 (arrow) was detected using the purified Pab.Application
TECT1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TECT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TECT1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TECT1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TCTN1; TECT1; Tectonic-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TECT1 |
|---|---|
| Alternative Names | TCTN1 TECT1 Tectonic-1 |
| Molecular Weight(MW) | 63570 Da |
| Function | Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals Modulates Hh signal transduction downstream of SMO and RAB23 (By similarity) |
| Tissue Specificity | This TECT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 260-290 from the Central region of human TECT1. |
| Cellular Localization | Cytoplasm, cytoskeleton, cilium basal body (By similarity). Secreted (Potential). Note=Despite the presence of a signal sequence, the full length protein might not be secreted (By similarity). Localizes at the transition zone, a region between the basal body and the ciliary axoneme (By similarity) |
| Entrez Gene | 79600 |
|---|---|
| Nucleotide Accession | NP_001076006 |
Application
Western blot analysis of TECT1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). TECT1 (arrow) was detected using the purified Pab.
Application
TECT1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TECT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TECT1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
