Application
Western blot analysis of TGFA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).TGFA (arrow) was detected using the purified Pab.Application
TGFA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TGFA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of TGFA (arrow) using rabbit polyclonal TGFA Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGFA gene.Application
TGFA Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TGFA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TGFA Protransforming growth factor alpha Transforming growth factor alpha EGF-like TGF TGF type 1 |
|---|---|
| Molecular Weight(MW) | 17006 Da |
| Function | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar |
| Tissue Specificity | Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines |
| Cellular Localization | Transforming growth factor alpha: Secreted, extracellular space |
| Entrez Gene | 7039 |
|---|
Application
Western blot analysis of TGFA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).TGFA (arrow) was detected using the purified Pab.
Application
TGFA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TGFA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of TGFA (arrow) using rabbit polyclonal TGFA Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TGFA gene.
Application
TGFA Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
