Application
Western blot analysis of THBS1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). THBS1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with THBS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of THBS1 Antibody (N-term) (Cat#AP8522a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
THBS1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | THBS1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FCIFIHCWB |
| WB:1:500~2000 IHC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TSP, TSP1 |
|---|---|
| Alternative Names | THBS1 TSP TSP1 Thrombospondin-1 |
| Molecular Weight(MW) | 129383 Da |
| Function | Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties |
| Tissue Specificity | This THBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~218 amino acids from the N-terminal region of human THBS1. |
| Entrez Gene | 7057 |
|---|
Application
Western blot analysis of THBS1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). THBS1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with THBS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of THBS1 Antibody (N-term) (Cat#AP8522a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
THBS1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:500~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
