Application
THOC4 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the THOC4 antibody detected the THOC4 protein (arrow).| Product Name | THOC4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALY, BEF, THOC4 |
|---|---|
| Alternative Names | ALYREF ALY BEF THOC4 THO complex subunit 4 Ally of AML-1 and LEF-1 Aly/REF export factor Transcriptional coactivator Aly/REF bZIP-enhancing factor BEF |
| Molecular Weight(MW) | 26888 Da |
| Function | Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. ALYREF/THOC4 in conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA |
| Tissue Specificity | This THOC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the Central region of human THOC4. |
| Cellular Localization | Nucleus. Nucleus speckle. Cytoplasm. Note=Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA |
| Entrez Gene | 10189 |
|---|---|
| Nucleotide Accession | NP_005773.3 |
Application
THOC4 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the THOC4 antibody detected the THOC4 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
