Application
Western blot analysis of THOC7 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). THOC7 (arrow) was detected using the purified Pab.Application
THOC7 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | THOC7 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NIF3L1BP1 |
|---|---|
| Alternative Names | THOC7 NIF3L1BP1 THO complex subunit 7 homolog Functional spliceosome-associated protein 24 Ngg1-interacting factor 3-like protein 1-binding protein 1 hTREX30 |
| Molecular Weight(MW) | 23743 Da |
| Function | Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4 |
| Tissue Specificity | This THOC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the C-terminal region of human THOC7. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Interaction with THOC5 is required for nuclear localization |
| Entrez Gene | 80145 |
|---|
Application
Western blot analysis of THOC7 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). THOC7 (arrow) was detected using the purified Pab.
Application
THOC7 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
