Application
Western blot analysis of TIA1 antibody (Center R184) in K562 cell line lysates (35ug/lane). TIA1 (arrow) was detected using the purified Pab.Application
TIA1 Antibody (Center R184) (RB18831) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TIA1 Antibody (Center R184) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TIA1 Antibody (Center R184) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TIA1 Antibody (Center R184) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TIA1 Nucleolysin TIA-1 isoform p40 RNA-binding protein TIA-1 T-cell-restricted intracellular antigen-1 p40-TIA-1 |
|---|---|
| Molecular Weight(MW) | 42963 Da |
| Function | Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis |
| Tissue Specificity | This TIA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176~206 amino acids from the Center region of human TIA1. |
| Cellular Localization | Cytoplasmic granule. Nucleus. Note=Accumulates in cytoplasmic stress granules (SG) following cellular damage |
| Entrez Gene | 7072 |
|---|
Application
Western blot analysis of TIA1 antibody (Center R184) in K562 cell line lysates (35ug/lane). TIA1 (arrow) was detected using the purified Pab.
Application
TIA1 Antibody (Center R184) (RB18831) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TIA1 Antibody (Center R184) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TIA1 Antibody (Center R184) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
