Application
Western blot analysis of TIMM8A Antibody (C-term) in NIH-3T3 cell line lysates (35ug/lane). TIMM8A (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with TIMM8A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using TIMM8A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TIMM8A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DDP, DDP1, TIM8A |
|---|---|
| Alternative Names | TIMM8A DDP DDP1 TIM8A Mitochondrial import inner membrane translocase subunit Tim8 A Deafness dystonia protein 1 X-linked deafness dystonia protein |
| Molecular Weight(MW) | 10998 Da |
| Function | Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Probably necessary for normal neurologic development |
| Tissue Specificity | Highly expressed in fetal and adult brain, followed by fetal lung, liver and kidney. Also expressed in heart, placenta, lung, liver, kidney, pancreas, skeletal muscle and heart |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side |
| Entrez Gene | 1678 |
|---|
Application
Western blot analysis of TIMM8A Antibody (C-term) in NIH-3T3 cell line lysates (35ug/lane). TIMM8A (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with TIMM8A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using TIMM8A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
