Application
Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35ug/lane). TIMP3 (arrow) was detected using the purified Pab.Application
Western blot analysis of TIMP3 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TIMP3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TIMP3 Metalloproteinase inhibitor 3 Protein MIG-5 Tissue inhibitor of metalloproteinases 3 |
|---|---|
| Molecular Weight(MW) | 24145 Da |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15 |
| Tissue Specificity | This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~148 amino acids from the Center region of human TIMP3. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix |
| Entrez Gene | 7078 |
|---|
Application
Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35ug/lane). TIMP3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of TIMP3 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab (1:60/250 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
