Application
Western blot analysis of TIRAP Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). TIRAP (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of TIRAP Antibody (C-term) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
TIRAP Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TIRAP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAL |
|---|---|
| Alternative Names | TIRAP MAL Toll/interleukin-1 receptor domain-containing adapter protein Adaptor protein Wyatt MyD88 adapter-like protein |
| Molecular Weight(MW) | 23883 Da |
| Function | Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6 |
| Tissue Specificity | Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 114609 |
|---|
Application
Western blot analysis of TIRAP Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). TIRAP (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of TIRAP Antibody (C-term) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
TIRAP Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
