Application
TLR9 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TLR9 antibody detected the TLR9 protein (arrow).Application
TLR9 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TLR9 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TLR9 Toll-like receptor 9 CD_antigen=CD289 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 115860 Da |
| Function | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response Controls lymphocyte response to Helicobacter infection |
| Tissue Specificity | Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein (By similarity). Endosome (By similarity) Lysosome (By similarity). Cytoplasmic vesicle, phagosome (By similarity). Note=Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist (By similarity) |
| Entrez Gene | 54106 |
|---|---|
| Nucleotide Accession | NP_059138.1 |
Application
TLR9 Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TLR9 antibody detected the TLR9 protein (arrow).
Application
TLR9 Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
