Application
TMEM66 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).Application
TMEM66 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).Application
TMEM66 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TMEM66 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SARAF, XTP3 |
|---|---|
| Alternative Names | TMEM66 SARAF XTP3 Store-operated calcium entry-associated regulatory factor HBV X-transactivated gene 3 protein HBV XAg-transactivated protein 3 Protein FOAP-7 Transmembrane protein 66 |
| Molecular Weight(MW) | 36975 Da |
| Function | Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)- dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions |
| Tissue Specificity | Highly expressed in macrophages. |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Translocates to the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-dependent manner following cytosolic Ca(2+) elevation |
| Entrez Gene | 51669 |
|---|---|
| Nucleotide Accession | NP_057211.4 |
Application
TMEM66 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).
Application
TMEM66 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).
Application
TMEM66 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
