Application
A. Western blot analysis using anti-TNF-? Rabbit Monoclonal on 10 ng recombinant protein on membrane.(Cat. #AJ1775b), dilution 1:500.| Product Name | TNF alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1085Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TNFA, TNFSF2 |
|---|---|
| Alternative Names | TNF TNFA TNFSF2 Tumor necrosis factor soluble form Cachectin |
| Molecular Weight(MW) | 25644 Da |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation |
| Tissue Specificity | A synthetic peptide corresponding to the extracellular portion of human TNF-α was used as immunogen. |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein Tumor necrosis factor, soluble form: Secreted C-domain 2: Secreted. |
| Entrez Gene | 7124 |
|---|
Application
A. Western blot analysis using anti-TNF-? Rabbit Monoclonal on 10 ng recombinant protein on membrane.(Cat. #AJ1775b), dilution 1:500.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
