Application
TNF Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the TNF antibody detected the TNF protein (arrow).Application
TNF Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TNF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TNFA, TNFSF2 |
|---|---|
| Alternative Names | TNF TNFA TNFSF2 Tumor necrosis factor Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 Tumor necrosis factor membrane form N-terminal fragment Intracellular domain 1 Intracellular domain 2 C-domain 1 C-domain 2 Tumor nec |
| Molecular Weight(MW) | 25644 Da |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation |
| Tissue Specificity | This TNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the C-terminal region of human TNF. |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein Tumor necrosis factor, soluble form: Secreted C-domain 2: Secreted. |
| Entrez Gene | 7124 |
|---|---|
| Nucleotide Accession | NP_000585.2 |
Application
TNF Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the TNF antibody detected the TNF protein (arrow).
Application
TNF Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
