A. Western blot analysis on recombinant TNF-beta using TNF-beta RabMab (Cat. #AJ1776a), dilution 1:5,000. (0.05ug of recombinant protein was loaded on the membrane).
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo
Tissue Specificity
A synthetic peptide corresponding to residues near the N-term of human TNF-β was used as immunogen.
Cellular Localization
Secreted. Membrane. Note=The homotrimer is secreted. The heterotrimer is membrane-associated
A. Western blot analysis on recombinant TNF-beta using TNF-beta RabMab (Cat. #AJ1776a), dilution 1:5,000. (0.05ug of recombinant protein was loaded on the membrane).
Application Notes
WB:1:5000
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.