Application
Western blot analysis on (A) Jurkat cells treated with TNF and TPA and (B) Daudi cell lysates using anti-TNFAIP3 RabMAb (Cat. #AJ1777a).Application
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TNFAIP3 RabMAb (Cat. #AJ1777a).| Product Name | TNFAIP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Putative DNA-binding protein A20, Zinc finger protein A20 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2663 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OTUD7C |
|---|---|
| Alternative Names | Putative DNA-binding protein A20 Zinc finger protein A20 |
| Molecular Weight(MW) | 89614 Da |
| Function | Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3 In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains Inhibitor of programmed cell death. Has a role in the function of the lymphoid system |
| Tissue Specificity | A synthetic peptide corresponding to residues in human TNFAIP3 was used as an immunogen. |
| Cellular Localization | Cytoplasm. Nucleus. Lysosome. |
| Entrez Gene | 7128 |
|---|
Application
Western blot analysis on (A) Jurkat cells treated with TNF and TPA and (B) Daudi cell lysates using anti-TNFAIP3 RabMAb (Cat. #AJ1777a).
Application
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TNFAIP3 RabMAb (Cat. #AJ1777a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
