Application
Western blot analysis of anti-TNFRSF14 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. TNFRSF14 (arrow) was detected using the purified Pab (1:250 dilution).Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TNFRSF14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TNFRSF14 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TNFRSF14 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFRSF14; HVEA; HVEM; Tumor necrosis factor receptor superfamily member 14; Herpes virus entry mediator A; Tumor necrosis factor receptor-like 2; CD_antigen=CD270; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HVEA HVEM |
|---|---|
| Alternative Names | TNFRSF14 HVEA HVEM Tumor necrosis factor receptor superfamily member 14 Herpes virus entry mediator A Tumor necrosis factor receptor-like 2 CD_antigen=CD270 Flags: Precursor |
| Molecular Weight(MW) | 30392 Da |
| Function | Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells |
| Tissue Specificity | Widely expressed, with the highest expression in lung, spleen and thymus |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Probable) |
| Entrez Gene | 8764 |
|---|
Application
Western blot analysis of anti-TNFRSF14 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. TNFRSF14 (arrow) was detected using the purified Pab (1:250 dilution).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TNFRSF14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TNFRSF14 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
