Application
TNFRSF1A Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TNFRSF1A antibody detected the TNFRSF1A protein (arrow).Application
TNFRSF1A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNFRSF1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of TNFRSF1A Antibody (N-term)(Cat#AP11261a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
TNFRSF1A Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TNFRSF1A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TNFAR, TNFR1 |
|---|---|
| Alternative Names | TNFRSF1A TNFAR TNFR1 Tumor necrosis factor receptor superfamily member 1A Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 p60 CD_antigen=CD120a Tumor necrosis factor receptor superfamily member 1A membrane form Tumor |
| Molecular Weight(MW) | 50495 Da |
| Function | Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase |
| Tissue Specificity | This TNFRSF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human TNFRSF1A. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Secreted. Note=A secreted form is produced through proteolytic processing |
| Entrez Gene | 7132 |
|---|---|
| Nucleotide Accession | NP_001056.1 |
Application
TNFRSF1A Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TNFRSF1A antibody detected the TNFRSF1A protein (arrow).
Application
TNFRSF1A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNFRSF1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of TNFRSF1A Antibody (N-term)(Cat#AP11261a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
TNFRSF1A Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
