Application
TNKS Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TNKS antibody detected the TNKS protein (arrow).Application
TNKS Antibody (N-term) (AP14867a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNKS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TNKS Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNKS; PARP5A; PARPL; TIN1; TINF1; TNKS1; Tankyrase-1; ADP-ribosyltransferase diphtheria toxin-like 5; Poly [ADP-ribose] polymerase 5A; TNKS-1; TRF1-interacting ankyrin-related ADP-ribose polymerase; Tankyrase I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PARP5A PARPL TIN1 TINF1 TNKS1 |
|---|---|
| Alternative Names | TNKS PARP5A PARPL TIN1 TINF1 TNKS1 Tankyrase-1 ADP-ribosyltransferase diphtheria toxin-like 5 Poly [ADP-ribose] polymerase 5A TNKS-1 TRF1-interacting ankyrin-related ADP-ribose polymerase Tankyrase I |
| Molecular Weight(MW) | 142039 Da |
| Function | Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1 |
| Tissue Specificity | Ubiquitous; highest levels in testis. |
| Cellular Localization | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Chromosome, centromere. Nucleus, nuclear pore complex. Chromosome, telomere (Potential). Cytoplasm, cytoskeleton, spindle pole. Note=Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1. Localizes to spindle poles at mitosis onset via interaction with NUMA1 |
| Entrez Gene | 8658 |
|---|---|
| Nucleotide Accession | NP_003738.2 |
Application
TNKS Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TNKS antibody detected the TNKS protein (arrow).
Application
TNKS Antibody (N-term) (AP14867a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNKS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
